Researchers at Tsukuba University discover a new group of carbonic anhydrase enzymes that do not require any metal ions for their activity, which improves our knowledge of the global carbon cycle and may be applicable to artificial photosynthesis

Tsukuba, Japan—Carbonic anhydrases are essential enzymes that are present in virtually all living things; all eight classes of carbonic anhydrases that have been identified to date need a metal ion to function. But now, researchers from Japan have discovered that metal is not crucial for all carbonic anhydrases.

In a study published this month in BMC Biology, researchers from the Faculty of Life and Environmental Sciences at the University of Tsukuba have described two members of the COG4337 protein family that are the first known examples of carbonic anhydrase enzymes that do not require a metal ion to function.

Carbonic anhydrases catalyze the conversion of carbon dioxide (CO₂) into bicarbonate (HCO₃‾), and vice versa. They are central to a wide range of physiological processes, including regulation of acid-base balance, respiration, and photosynthesis, and are crucial for all carbon-based life, from bacteria to humans. All previously identified classes of carbonic anhydrase contain a metal cofactor—zinc, cadmium, cobalt, or manganese—that is essential for the activity of these enzymes, so when the researchers at the University of Tsukuba identified that the COG4337 proteins were similar to known carbonic anhydrases, they expected metal ions to be important for these new proteins too.

"Because the active site of most carbonic anhydrases contains a metal ion that facilitates interconversion between CO₂ and HCO₃‾, we were surprised to find that the COG4337 proteins did not require any of the eight different metals we tested, and actually were less functional in the presence of zinc," lead author Professor Yoshihisa Hirakawa says.

When the researchers made a model of COG4337 protein structure, they found that there was an active site, or pocket, that may hold on to CO₂ while the enzyme converts it to HCO₃‾. Interestingly, unlike other carbonic anhydrases, these novel enzymes don't seem to carry out the reverse reaction of converting HCO₃‾ to CO₂. In addition, the investigators saw that these COG4337 proteins tend to congregate in the plastids and mitochondria of the microalga Bigelowiella natans, which is where CO₂ metabolism takes place.

"These proteins are expressed by many cyanobacteria and eukaryotic microalgae that live in environments with fairly diverse metal contents. It is possible that this enzyme evolved in an ancestral microbe to adapt to metal-poor environments, such as the open ocean," Professor Hirakawa explains.

Given the widespread expression of carbonic anhydrases in ecologically important species of microalgae, this novel type of metal-independent carbonic anhydrases may play an important role in the global carbon cycle. Understanding more about how these enzymes work could also be useful for artificial photosynthesis, an important source of renewable energy.

Original Paper

The article, "Characterization of a novel type of carbonic anhydrase that acts without metal cofactors," was published in BMC Biology as DOI:10.1186/s12915-021-01039-8.

Correspondence

Assistant Professor HIRAKAWA Yoshihisa
Faculty of Life and Environmental Sciences, University of Tsukuba

Related Link

Faculty of Life and Environmental Sciences